User’s Guide

Contents:

• Introduction
• Principles of Operation
  • Overview
  • Manager
  • Devices
  • Modules
  • Tasks
  • Data Handling
  • Coordinate systems in ACQ4
• Hardware Recommendations and Requirements
  • Basic Patch Clamp Electrophysiology
  • Patch Clamp Automation
  • Stage and Manipulator Accuracy
  • Basic Microscopy Imaging
  • Laser Scanning Imaging
  • Photostimulation
• Installation
  • Improving performance with GPU
  • Starting ACQ4
  • Legacy Python 2 support
• Configuration
  • .cfg File Syntax
  • Configuration Structure
• Configuring and Calibrating Hardware
  • Coordinate Systems
  • 1. Camera Scale
  • 2. Objective Scaling
  • 3. Camera Orientation
  • 4. Stage Orientation
  • 5. Fine Tuning the Stage Orientation
  • 6. Set the global coordinate origin
  • 7. Objective offset calibration
  • 8. Initial manipulator calibration
  • 9. Set home and cleaning positions for each manipulator
  • 10. Configure pipette approach angle
• Data Management
  • Hierarchical File Storage
  • Meta Information
  • User-defined Folder Types
  • Notes and Logs
  • MetaArray Files
• User interface elements
  • Plots and interactive views
  • Image viewers
  • Function generators
• Modules
  • The Manager module
  • The Data Manager Module
  • The Task Runner module
  • The Camera Module
  • The Patch module
  • Imager Module
  • Console Module
• Devices
  • NiDAQ Devices
  • Generic DAQ Devices
  • Camera Devices
  • Patch-Clamp Amplifiers
  • Movable Stages
  • Scanner Devices
  • Lasers
  • Microscope Devices
  • Trigger Devices
  • Digital Switch
  • Optomechanical devices
• Tutorials
  • Patching a Cell
  • Record an I/V Curve
  • Keeping Data Organized
  • Troubleshooting Table Vibration
  • Troubleshoot Electrical Noise

Manager

Works behind the scenes to manage devices and modules; runs protocols on behalf of modules.